FastQCFastQC Report
Sat 18 Mar 2023
SRR4895433_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895433_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68484
Sequences flagged as poor quality0
Sequence length150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA7111.0381987033467672No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT6881.0046142164593188No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGAAAAAAAAAAAAAAAA5720.8352315869400152No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA5720.8352315869400152No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5340.7797441738216225No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2650.38695169674668534No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTCTTGCTTCAAC2540.3708895508439928No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA2040.2978797967408446No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCCCGATGCTGACGGCTTA1840.2686758950995853No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCCTGGAGATTGTC1650.24093218854038898No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCATCTCCTCTAAC1590.2321710180480112No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCACTTAGATATTTTAAAG1550.22633023771975935No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA1550.22633023771975935No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGGACCGCCGCTTTCGCTC1470.21464867706325563No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCTTCTTTGAGAGTTGTTG1350.19712633607850008No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCCTGGAGATTGTCT1350.19712633607850008No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGATCTTCCGACGACATTG1340.19566614099643712No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTGCTTCGGCTGCTTGAGG1200.17522340984755563No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTCTTGCTTCAACA1190.17376321476549267No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAATTATCAATCAAGCTCC1180.1723030196834297No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACGAGGATCCATTGGAGGG1180.1723030196834297No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGATACAGTAGGATTTATTC1150.1679224344372408No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTAGGATTTATTCAGGATC1140.16646223935517784No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCCAAAAAAAAAAAAAAA1140.16646223935517784No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGAAAAAACAGTGCTTCGG1110.16208165410898898No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGCGGGACATGAAAAAACA1080.15770106886280008No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCATCTCCTCTAACT1040.15186028853454822No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCAGGATCTTCCGACGAC1040.15186028853454822No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACATTCCAAAAAAAAAAAAA960.14017872787804453No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGATTTATTCAGGATCTTC950.13871853279598156No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCAATCAAGCTCCCCGAGG900.1314175573856667No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCTCCCCGAGGAGCGGAC880.12849716722154078No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCCGCTCAACGCTTGAGG850.1241165819753519No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCCCAGCACTTAGATATT840.12265638689328894No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCTTGGTAGGGATAGAT830.12119619181122597No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA830.12119619181122597No Hit
AAGCAGTGGTATCAACGCAGAGAACATGGGGAAAAAAAAAAAAAAAAAAA810.11827580164710004No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACTTGTCATATCGCGCACG790.11535541148297412No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGCTTCCCATCTTCTTTG770.1124350213188482No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCTCCAATAGCGTATATTA750.10951463115472226No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAGAAAAAAAAAAAAAAAAAAA740.1080544360726593No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAACCCCAGCACTTAGATA730.10659424099059633No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGTGAGGCTCTCTCATTGG720.10513404590853338No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCTTCCCATCTTCTTTGAG720.10513404590853338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT37900.0142.100271
CAGTGGT38600.0139.336794
AGTGGTA38650.0139.156545
AGCAGTG38850.0138.440162
GCAGTGG38900.0138.26223
GTGGTAT38950.0138.084726
GGTATCA38750.0137.682598
GTATCAA39000.0136.984629
TGGTATC39250.0135.928677
TGGTATA451.1294105E-896.07
GTATAAA554.546382E-878.5454569
GGTATAA608.3091436E-872.08
ATGGGTA502.6211637E-928.80000125-29
GGAAGGT353.973504E-628.80000125-29
TGGGACG255.359574E-428.80000125-29
GGGGTAA200.00627763428.825-29
GGGAACG552.2919266E-1028.825-29
TCAGTGA200.00627763428.890-94
CAACGCA38600.028.2777210-14
ATCAACG38800.028.0577310-14
Produced by FastQC (version 0.11.5)