FastQCFastQC Report
Sat 18 Mar 2023
SRR4895432_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895432_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences825819
Sequences flagged as poor quality0
Sequence length150
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCA826400.0143.891571
TACGCAA826650.0143.848072
ACGCAAT308250.0143.809273
ACGCAAG285500.0143.13873
CGCAATG106600.0143.11814
CGCAAGT104650.0143.101754
ACGCAAA235000.0143.015733
CGCAATA90500.0142.802784
CGCAATT112350.0142.778534
CGCAAAG77650.0142.419864
CGCAAGA80850.0142.39324
CGCAAGG100700.0142.280184
GCAATGG39850.0140.92475
GCAATTG39600.0140.905295
CGCAAAT90250.0140.565734
GCAATGA28750.0140.490135
GCAATAT33400.0140.331565
GCAATGT39850.0140.2025
GCAAGGT39850.0140.021335
GCAAGTA32550.0140.014665
Produced by FastQC (version 0.11.5)