FastQCFastQC Report
Sat 18 Mar 2023
SRR4895432_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895432_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences825819
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42790.5181522827641407No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT21020.2545351947581734No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA19890.2408518089314971No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG14210.1720716040682038No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT13690.1657748247497333No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA12250.1483375897139688No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG11570.1401033398359689No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGAAAAAAAAAAAAAAAA11150.13501747961720426No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC11040.13368546860752778No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTCTTGCTTCAAC8330.10086956100549879No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT264550.0139.189771
GTGGTAT269550.0136.385946
GGTATCA269950.0136.130518
AGTGGTA270250.0136.032675
GTATCAA271100.0135.977989
AGCAGTG270600.0135.73192
CAGTGGT271350.0135.454684
TGGTATC271500.0135.379857
GCAGTGG275350.0133.338123
CGTTATC400.006217199454.0027281
CGTGGTA1102.536918E-432.7269444
ATCAACG270150.027.90421510-14
AGAGTAC270300.027.81947315-19
CAGAGTA270700.027.77836815-19
CAACGCA272750.027.75436610-14
TCAACGC272850.027.67031110-14
TACATGG269800.027.66821520-24
GCAGAGT272650.027.49519215-19
GTACATG272050.027.46584920-24
AACGCAG275650.027.42580610-14
Produced by FastQC (version 0.11.5)