FastQCFastQC Report
Sat 18 Mar 2023
SRR4895431_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895431_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1429254
Sequences flagged as poor quality0
Sequence length150
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGAA1430550.0143.862722
TGCGAAT535750.0143.684483
TGCGAAG481350.0143.634583
GGTGCGA1433250.0143.59171
GCGAATA157100.0143.489464
TGCGAAA414250.0143.472173
GCGAATT194750.0143.402074
GCGAATG183800.0143.327654
GCGAAAG147650.0142.872064
GCGAAGT178150.0142.861984
GCGAAGA136750.0142.730014
CGAAGTA56000.0142.707925
GCGAAAT152600.0142.672534
CGAATTG67500.0142.606965
CGAATGG65100.0142.113455
CGAATGT70200.0141.942375
CGAATGA50150.0141.840125
GCGAAGG169150.0141.737674
CGAAGAT48750.0141.482915
CGAAGTT67050.0141.309115
Produced by FastQC (version 0.11.5)