FastQCFastQC Report
Sat 18 Mar 2023
SRR4895431_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895431_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1429254
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA79040.5530157690655405No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC22980.16078317779764828No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT21480.15028819230171825No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG19890.1391635076760324No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA18800.1315371515489899No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGAAAAAAAAAAAAAAAA17430.12195173146270712No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA15740.11012738113729259No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT14480.10131159332071135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT411600.0139.689871
GTGGTAT415600.0137.845816
GGTATCA416350.0137.58028
TGGTATC417100.0137.332817
GTATCAA420100.0136.951939
AGCAGTG419650.0136.478322
AGTGGTA420800.0136.142385
CAGTGGT421400.0135.948534
GCAGTGG425100.0134.72863
ATCAACG417200.028.14337210-14
AGAGTAC415700.028.13407915-19
TCAACGC418600.028.09396710-14
CAGAGTA417200.028.05018215-19
TACATGG416250.027.91701920-24
CAACGCA423500.027.85051310-14
GTACATG418150.027.80049920-24
GCAGAGT421000.027.68412615-19
AGTACAT421800.027.65893220-24
ACATGGG419650.027.59475520-24
CGCAGAG425050.027.55924215-19
Produced by FastQC (version 0.11.5)