FastQCFastQC Report
Sat 18 Mar 2023
SRR4895430_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895430_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1250642
Sequences flagged as poor quality0
Sequence length150
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGAAC1253400.0143.671341
GAGAACA1254100.0143.591142
AGAACAT450100.0142.87333
AGAACAG449750.0142.760383
GAACATG155550.0142.697024
GAACAGT165700.0142.037774
GAACAAT131650.0141.696124
AGAACAA362950.0141.414263
GAACATA132400.0141.328494
GAACATT164650.0141.150744
GAACAGG155250.0141.071414
GAACAGA131950.0140.664634
GAACAAG133650.0140.383744
AACATGG58200.0137.807745
AACATTG62050.0137.263285
ACATAGG19200.0137.243336
AACAGTG57900.0136.905265
AACATGA44800.0136.600515
AACAGTA50450.0136.144035
AACATAG51000.0136.087515
Produced by FastQC (version 0.11.5)