FastQCFastQC Report
Sat 18 Mar 2023
SRR4895430_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895430_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1250642
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA65610.5246105600163755No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT26960.21556928361593486No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA24310.1943801663465644No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT22990.18382558717842515No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC20670.16527511470108952No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG16470.13169236280246466No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG15810.12641507321839504No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC15050.12033819430340577No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT286100.0139.891951
GGTATCA288950.0138.132898
GTGGTAT289700.0137.974096
GTATCAA290300.0137.738519
TGGTATC290450.0137.394737
AGCAGTG292150.0136.748552
CAGTGGT293200.0136.30254
AGTGGTA294350.0135.794435
GCAGTGG300250.0133.083393
ATCAACG289750.028.10190410-14
AGAGTAC290350.028.05915615-19
CAGAGTA291400.027.94322815-19
TCAACGC292050.027.93975310-14
TACATGG289100.027.92416420-24
GTACATG291600.027.75883520-24
AGTACAT293900.027.60039720-24
CAACGCA297300.027.56260310-14
GCAGAGT295050.027.49994315-19
GAGTACA290650.027.3541120-24
CGCAGAG299200.027.22438415-19
Produced by FastQC (version 0.11.5)