FastQCFastQC Report
Sat 18 Mar 2023
SRR4895429_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895429_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1413712
Sequences flagged as poor quality0
Sequence length150
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTA1414850.0143.878042
TCGGTAT527900.0143.654133
GCTCGGT1417300.0143.629331
TCGGTAG462250.0143.574543
TCGGTAA426100.0143.521973
CGGTAGA129750.0143.051774
CGGTAAT158150.0142.948034
CGGTATA151950.0142.857914
CGGTATG182450.0142.811234
CGGTAGT167950.0142.75194
CGGTAAG143500.0142.740774
CGGTATT196050.0142.562844
CGGTAGG166350.0142.307134
CGGTAAA127100.0141.4464
GGTAGTA54100.0135.743995
GGTATAG53900.0135.312625
GGTAATA46950.0134.947465
GGTATTA58750.0134.55885
GGTATGA52900.0133.78755
GGTATAA46500.0132.692255
Produced by FastQC (version 0.11.5)