FastQCFastQC Report
Sat 18 Mar 2023
SRR4895429_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895429_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1413712
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA68690.4858839707097344No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGAAAAAAAAAAAAAAAA22800.16127754450694343No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG21870.15469911834942338No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA21800.154203967993481No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC17100.12095815838020757No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT15920.1126113380943219No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA15000.10610364770193645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT504850.0139.829321
GGTATCA511300.0137.727438
GTGGTAT511850.0137.537256
AGCAGTG512700.0137.337312
TGGTATC512700.0137.337317
AGTGGTA513550.0137.081945
GTATCAA515450.0137.023649
CAGTGGT515000.0136.709984
GCAGTGG518350.0135.74313
ATCAACG512100.028.16323310-14
CAACGCA514800.028.12740910-14
TCAACGC513800.028.10928510-14
AGAGTAC513100.028.01913515-19
CAGAGTA513800.027.98376515-19
TACATGG511300.027.90793820-24
GTACATG513400.027.81341720-24
AACGCAG520400.027.80812810-14
GCAGAGT515450.027.80479215-19
CGCAGAG517850.027.78437415-19
AGTACAT517200.027.709320-24
Produced by FastQC (version 0.11.5)