FastQCFastQC Report
Sat 18 Mar 2023
SRR4895428_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895428_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences824478
Sequences flagged as poor quality0
Sequence length150
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACACA826400.0143.650421
GACACAC828450.0143.294952
ACACACG270550.0141.967773
ACACACT313100.0141.87473
CACACGT96400.0140.70414
CACACGG94550.0140.563644
CACACTA95000.0139.897834
ACACGGT36800.0139.686115
CACACTG102650.0139.571584
CACACGA81950.0139.50974
ACACGAT30200.0139.222295
CACACTT120650.0138.559954
CACACAG87900.0138.011064
CACACAT91500.0138.010254
ACACTGT38900.0137.882635
CACGGAT10550.0137.84846
ACACGTA30050.0137.760995
ACACGTG32200.0137.729745
ACACGGA29450.0137.38965
ACACTAG32450.0137.334235
Produced by FastQC (version 0.11.5)