FastQCFastQC Report
Sat 18 Mar 2023
SRR4895428_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895428_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences824478
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34830.4224491132571154No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC26950.3268734884375326No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG24870.30164540472881024No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA22310.27059545554884423No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT19430.23566426272138252No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCGGTGGCTCA18200.2207457324513207No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG17680.21443871152414012No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC11940.14481890359718513No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC10050.12189530830416336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA157200.0139.869748
AAGCAGT158450.0138.99351
GTGGTAT158400.0138.764656
GTATCAA159500.0138.168769
TGGTATC160350.0137.077157
CAGTGGT160300.0137.0754
AGCAGTG161750.0135.890722
AGTGGTA162500.0135.219215
GCAGTGG165150.0133.093083
ATCAACG157200.028.45025410-14
AGAGTAC157500.028.33206715-19
CAGAGTA158450.028.14402415-19
TACATGG157350.028.10284620-24
TCAACGC159550.028.06731210-14
GTACATG158500.027.98979220-24
GCAGAGT159950.027.84408415-19
AGTACAT160500.027.8114720-24
GAGTACA158100.027.70541220-24
CAACGCA162100.027.66131610-14
ACATGGG160350.027.44237120-24
Produced by FastQC (version 0.11.5)