FastQCFastQC Report
Sat 18 Mar 2023
SRR4895427_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895427_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1410584
Sequences flagged as poor quality0
Sequence length150
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATCG474700.0143.49283
CTCTATC1416000.0143.439092
CCTCTAT1416500.0143.388461
CTATCGA134550.0143.083654
CTATCAG139400.0142.753754
CTATCTG174900.0142.676044
TCTATCA422900.0142.631353
CTATCGT185800.0142.559574
CTATCGG156200.0142.333974
CTATCTA149850.0141.783194
TCTATCT525100.0141.717253
CTATCAT161500.0141.630574
TATCGAA39450.0141.255785
TATCGGA43550.0141.182885
TATCGAT54150.0140.935275
TATCAGG47200.0140.790055
CTATCAA123950.0140.682434
ATCGATA16550.0140.51316
ATCGGAT15750.0140.336326
TATCGGG55000.0140.328025
Produced by FastQC (version 0.11.5)