FastQCFastQC Report
Sat 18 Mar 2023
SRR4895427_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895427_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1410584
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA64600.4579663458539158No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG63000.44662352614236367No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA42050.29810348054422847No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC38110.2701717870045315No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA22850.16198964400560334No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT21100.14958343494609325No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT18260.1294499299580883No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT17100.121226385667213No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT295650.0139.758471
GTGGTAT298950.0137.661796
GGTATCA299550.0137.55438
TGGTATC301250.0136.754177
GTATCAA303050.0136.488339
AGCAGTG303200.0135.80342
AGTGGTA303350.0135.712525
CAGTGGT303400.0135.690174
GCAGTGG309200.0133.051733
ATCAACG299450.028.19807210-14
TCAACGC301500.028.03977610-14
AGAGTAC301850.027.92656115-19
CAACGCA304100.027.90894910-14
TACATGG299800.027.80689420-24
CAGAGTA304250.027.72046915-19
GTACATG301650.027.70319220-24
AGTACAT305250.027.52743520-24
CGCAGAG306450.027.52146315-19
GCAGAGT306300.027.47852715-19
ACATGGG301550.027.41152220-24
Produced by FastQC (version 0.11.5)