FastQCFastQC Report
Sat 18 Mar 2023
SRR4895426_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895426_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2287113
Sequences flagged as poor quality0
Sequence length150
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAATC2291150.0143.739721
CTAATCC2291250.0143.733442
TAATCCG786250.0143.72873
AATCCGG278250.0143.32154
AATCCGT294900.0143.066514
AATCCGA214300.0142.751174
TAATCCA670850.0142.706383
TAATCCT840400.0142.63213
ATCCGTA87800.0142.600235
ATCCGAT80850.0142.035165
AATCCTG293300.0142.005924
ATCCTAG89050.0141.972995
AATCCTA243600.0141.954934
AATCCAG234050.0141.748664
ATCCGGA73000.0141.725855
ATCCGGT104600.0141.722825
ATCCTGA72650.0141.41765
ATCCGTG103700.0141.217135
AATCCAT245200.0141.175454
ATCCGAG71850.0140.98815
Produced by FastQC (version 0.11.5)