FastQCFastQC Report
Sat 18 Mar 2023
SRR4895426_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895426_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2287113
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA121030.5291824234307618No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG100250.43832552217577353No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC68290.2985860340088137No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA40100.17533020887030942No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTGGAGGGCAAGTCTGGTG34830.15228805922575753No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCCGCGGTAATTCCAGCTC30220.13213164369228805No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCGCGCAAATTACCCACT25320.1107072540797066No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGACTCTTTCGAGGCCCTG24450.10690333184237072No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCTCCAATAGCGTATATTA24100.1053730182986149No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACGAGGATCCATTGGAGGG23630.10331802582557136No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG23370.10218122147878134No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT23290.10183143552592284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT803000.0139.623781
GTGGTAT811150.0137.889476
GGTATCA812250.0137.747068
TGGTATC812400.0137.70397
GTATCAA816050.0137.40569
AGTGGTA816000.0137.114035
CAGTGGT816300.0137.028354
AGCAGTG817650.0136.778692
GCAGTGG828350.0134.968413
ATCAACG813250.028.11223410-14
CAACGCA816750.028.104610-14
TCAACGC814550.028.10272810-14
AGAGTAC817150.027.93085315-19
CAGAGTA818250.027.89330315-19
TACATGG811950.027.81795720-24
GTACATG814400.027.78024520-24
AACGCAG827400.027.73936810-14
CGCAGAG823650.027.71917315-19
AGTACAT822750.027.64882720-24
GCAGAGT824150.027.62722615-19
Produced by FastQC (version 0.11.5)