FastQCFastQC Report
Sat 18 Mar 2023
SRR4895425_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895425_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1589928
Sequences flagged as poor quality0
Sequence length150
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAAC1592600.0143.750152
CCGACAA1593100.0143.705051
GACAACG560050.0143.58293
GACAACT570500.0143.476873
ACAACGG201000.0142.597324
ACAACGT207400.0142.570984
GACAACA465600.0142.432483
CAACGGT77400.0141.761815
ACAACTG193850.0141.691544
CAACTAG61900.0141.31915
ACAACGA156450.0140.864974
ACAACTA177300.0140.623864
CAACGTA64000.0140.394425
CAACGAT57650.0139.748125
CAACGTT76150.0139.645145
CAACTGA52350.0139.318245
CAACGTG71300.0139.046375
CAACTGG70950.0138.920495
ACAACAG166300.0138.452714
CAACGGA58950.0138.376195
Produced by FastQC (version 0.11.5)