FastQCFastQC Report
Sat 18 Mar 2023
SRR4895425_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895425_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1589928
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA79590.5005887059036636No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG39160.2463004614045416No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC30990.19491448669373707No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT22330.14044661141888187No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTACTTGCCGGAGGTGC21200.13333937134260168No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTGACTGGGCGCCGGCTG21170.13315068355296591No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA21150.13302489169320875No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA20030.12598054754680715No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGACTCCTGGACTGCATTAG18760.11799276445222677No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGGCAGGATCACAGGCTC18580.11686063771441224No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAATTGTATTGAAAA18250.11478507202841891No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGATGCAAATGTTTTGAAA17410.1095018139186177No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATACGGAAAAAATTGTATTG16080.1011366552447658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT538500.0139.806471
GGTATCA545100.0137.563498
GTGGTAT545250.0137.529976
GTATCAA548650.0137.185189
TGGTATC547050.0137.064297
AGCAGTG548600.0136.694462
AGTGGTA549100.0136.57885
CAGTGGT549700.0136.469014
GCAGTGG554000.0135.310063
TGTATCA10600.028.5274858
ATCAACG544950.028.21821210-14
TCAACGC547400.028.15767710-14
CAACGCA550900.028.0415210-14
AGAGTAC548550.028.02322615-19
CAGAGTA550550.027.91357815-19
TACATGG546450.027.86458620-24
GTACATG548650.027.83159320-24
AACGCAG556550.027.7309710-14
AGTACAT553200.027.71721520-24
GCAGAGT553250.027.69666515-19
Produced by FastQC (version 0.11.5)