FastQCFastQC Report
Sat 18 Mar 2023
SRR4895424_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895424_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1707674
Sequences flagged as poor quality0
Sequence length150
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATCCT1715300.0143.349611
ATCCTCG586400.0143.206043
CATCCTC1718500.0143.082692
ATCCTCA504750.0142.593953
ATCCTCT630750.0142.108443
TCCTCGA162800.0141.692184
TCCTCAG175150.0140.292334
TCCTCGG209400.0139.96914
CCTCGTA65700.0139.49895
CCTCGGA58300.0139.175615
TCCTCTA191250.0139.173254
TCCTCGT226650.0138.623474
CCTCGAT60950.0137.967535
CCTCTAG66200.0137.90155
CTCGAAT20250.0137.592166
CTCGGAT23450.0136.930376
TCCTCAA152350.0136.761494
CTCGATA18800.0136.715626
CCTCGAA54900.0136.647965
CCTCAGT67650.0136.542115
Produced by FastQC (version 0.11.5)