FastQCFastQC Report
Sat 18 Mar 2023
SRR4895424_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895424_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1707674
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA91000.5328885958326941No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG77780.4554733514710653No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC62090.363593988079692No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA60790.3559812938535107No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT23190.13579875315780413No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA22240.13023563045405623No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGCGACCTCAGATCAGACGT19400.11360482152916775No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG18510.10839305394355128No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT393050.0137.717671
GTGGTAT396800.0135.994836
GGTATCA397250.0135.695798
GTATCAA399700.0135.206279
TGGTATC399300.0134.98117
AGTGGTA401850.0134.303735
AGCAGTG402050.0134.205032
CAGTGGT402250.0134.152274
GCAGTGG411500.0131.123033
ATCAACG395850.027.8389910-14
TCAACGC398050.027.7393910-14
AGAGTAC398950.027.59756715-19
CAGAGTA400000.027.53952215-19
GTACATG397200.027.5204120-24
TACATGG396900.027.49041720-24
CAACGCA403850.027.40161910-14
AGTACAT402850.027.26311720-24
GCAGAGT404050.027.1815115-19
ACATGGG399850.027.07511920-24
GAGTACA399700.026.90874720-24
Produced by FastQC (version 0.11.5)