FastQCFastQC Report
Sat 18 Mar 2023
SRR4895423_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895423_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1026815
Sequences flagged as poor quality0
Sequence length150
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTTAC1028950.0143.692892
CACCTTA1030150.0143.525511
CCTTACG336550.0143.245033
CTTACGT134350.0143.189934
CTTACGA92400.0142.824984
CCTTACT392150.0142.635183
CTTACGG109800.0142.354494
CCTTACA305400.0142.343543
CTTACTG123850.0142.24984
CTTACTA116300.0142.136584
CTTACAG97250.0141.846824
TTACGAT38300.0140.986055
TTACGGA30700.0140.476015
TTACGTA39100.0140.126925
TTACGGT45800.0139.749425
CTTACAA89450.0139.486424
TTACTAG39550.0139.260735
TTACGTG42600.0139.092585
TTACGAG27150.0138.95535
TTACGAA28950.0138.77125
Produced by FastQC (version 0.11.5)