FastQCFastQC Report
Sat 18 Mar 2023
SRR4895423_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895423_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1026815
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54690.5326178522908216No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG47260.46025817698416954No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC39300.3827369097646606No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT25000.24347131664418614No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA23920.2329533557651573No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA21950.21376781601359546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT204150.0138.674361
GGTATCA204650.0137.941838
GTGGTAT204750.0137.663486
GTATCAA207000.0136.96719
TGGTATC206900.0136.302557
CAGTGGT208000.0135.616354
AGCAGTG208550.0135.127172
AGTGGTA208600.0135.088215
GCAGTGG212500.0132.683173
ATCAACG205150.028.19494410-14
AGAGTAC205900.027.98761615-19
CAGAGTA206000.027.96004715-19
TCAACGC207600.027.90381610-14
TACATGG205800.027.84137220-24
CAACGCA209750.027.75509310-14
GTACATG206650.027.71291520-24
AGTACAT208800.027.51031520-24
GCAGAGT208850.027.4612915-19
GAGTACA205700.027.27386720-24
CGCAGAG211600.027.24730515-19
Produced by FastQC (version 0.11.5)