FastQCFastQC Report
Sat 18 Mar 2023
SRR4895422_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895422_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2500610
Sequences flagged as poor quality0
Sequence length150
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGAGC2506200.0143.674672
CAAGGAG2509500.0143.485731
AGGAGCT945850.0143.334173
GGAGCTA269500.0143.248384
AGGAGCA704900.0142.88313
AGGAGCG863500.0142.820773
GGAGCAT260100.0142.529334
GGAGCTT371300.0142.096134
GAGCTAT105450.0141.743275
GAGCTTA101050.0141.716435
GAGCGTA97900.0141.569525
GAGCATA76050.0141.440285
GAGCGAT92900.0141.43895
GAGCTAA75550.0141.423365
GGAGCGA245300.0141.178734
GAGCTAG91800.0141.094535
GGAGCGT334450.0141.090234
GGAGCAA217600.0140.952384
GGAGCTG313600.0140.277134
GAGCTGA83700.0140.125555
Produced by FastQC (version 0.11.5)