FastQCFastQC Report
Sat 18 Mar 2023
SRR4895422_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895422_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2500610
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA128620.5143544975026093No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG48590.19431258772859422No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC43240.17291780805483462No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT806750.0140.034551
GTGGTAT814200.0138.29346
GGTATCA814950.0138.157298
TGGTATC815650.0138.00347
GTATCAA821550.0137.573219
AGCAGTG821700.0136.934752
AGTGGTA823650.0136.715475
CAGTGGT823500.0136.705384
GCAGTGG830850.0135.366063
CAACGCA817300.028.337810-14
TCAACGC816250.028.28428310-14
ATCAACG816900.028.21065910-14
AGAGTAC818350.028.12394315-19
CAGAGTA819950.028.05852915-19
TACATGG815150.028.00736220-24
CGCAGAG822250.027.9730415-19
GTACATG817300.027.96892720-24
AACGCAG830350.027.86815610-14
AGTACAT824000.027.83937520-24
GCAGAGT824700.027.82183815-19
Produced by FastQC (version 0.11.5)