FastQCFastQC Report
Sat 18 Mar 2023
SRR4895421_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895421_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2647321
Sequences flagged as poor quality0
Sequence length150
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGCT2652050.0143.73461
TTGGCTC2662600.0143.165072
TGGCTCA749600.0143.15693
GGCTCTA291650.0142.733544
GGCTCAT281550.0142.63724
TGGCTCT1060000.0142.389573
GGCTCGA239450.0142.218524
TGGCTCG862100.0141.996553
GGCTCAA217750.0141.975624
GCTCGTA95850.0141.889335
GGCTCAG254500.0141.701074
GGCTCGT335250.0141.093324
GCTCGAA70450.0140.926675
GCTCTAT108900.0140.81915
GCTCGAT90550.0140.732595
GCTCAGT98350.0140.698325
GCTCAAT85050.0140.352485
GCTCATA85350.0140.280915
GCTCATG92050.0140.165
GGCTCTG363650.0140.032854
Produced by FastQC (version 0.11.5)