FastQCFastQC Report
Sat 18 Mar 2023
SRR4895421_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895421_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2647321
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130320.4922712432681945No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG111750.422124857544665No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC78260.2956196094089081No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA43930.16594134220972825No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT40290.15219159293489531No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTGGAGGGCAAGTCTGGTG34040.12858282014156952No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC31460.11883711873248465No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA30420.11490861893967526No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCGCGCAAATTACCCACT29940.11309546518914783No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGACTCTTTCGAGGCCCTG28870.10905364328693044No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCCGCGGTAATTCCAGCTC27480.10380305221769479No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGAAAAAAAAAAAAAAAA27380.10342531185300158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT968250.0140.329481
GTGGTAT981700.0137.966316
GGTATCA983700.0137.737058
TGGTATC983150.0137.71897
GTATCAA989050.0137.37789
AGTGGTA986000.0137.364645
AGCAGTG987150.0137.278052
CAGTGGT991450.0136.63864
GCAGTGG994400.0136.226493
CAACGCA986700.028.23273310-14
ATCAACG984100.028.18295110-14
TCAACGC986500.028.15963710-14
AGAGTAC987000.028.05200215-19
CAGAGTA989300.027.9751415-19
AACGCAG996900.027.9395310-14
TACATGG983650.027.88592320-24
CGCAGAG992550.027.88498915-19
GTACATG987450.027.81360820-24
GCAGAGT994450.027.7549715-19
AGTACAT995450.027.70870220-24
Produced by FastQC (version 0.11.5)