FastQCFastQC Report
Sat 18 Mar 2023
SRR4895420_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895420_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1303543
Sequences flagged as poor quality0
Sequence length150
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCATTC1306000.0143.717731
CATTCCG425750.0143.401493
TCATTCC1308900.0143.39932
CATTCCA384750.0142.646063
ATTCCGA122250.0142.638824
ATTCCGG142050.0142.422164
CATTCCT502600.0142.217073
ATTCCGT163500.0141.87974
ATTCCTG163150.0141.831044
ATTCCAG128850.0141.31134
ATTCCTA150850.0140.747914
ATTCCAT142500.0140.507224
TCCGTAT17350.0137.768886
TTCCGTA50450.0137.714175
TTCCGAG42000.0137.479375
TCCGATA14250.0137.425256
TTCCGAT46650.0137.357015
TTCCGGA42850.0136.432455
TTCCTAG57350.0136.209935
ATTCCTT197950.0135.955344
Produced by FastQC (version 0.11.5)