FastQCFastQC Report
Sat 18 Mar 2023
SRR4895419_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895419_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1700969
Sequences flagged as poor quality0
Sequence length150
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGA1702000.0143.903852
GCTAACG1702050.0143.899611
TAACGAG594250.0143.77463
TAACGAT611100.0143.651033
TAACGAA497100.0143.485753
AACGATA183350.0143.168084
ACGATAG64700.0143.102465
AACGATG211650.0143.074234
AACGAGT206900.0142.913964
AACGATT217750.0142.736254
AACGAAT175750.0142.599874
AACGAGA167600.0142.188484
AACGAGG223100.0142.056434
AACGAAG181200.0142.045754
ACGATGA56450.0141.952045
ACGAGTA63950.0141.853625
ACGATTG76350.0141.729545
ACGAGTT75250.0141.696445
ACGAGGT81000.0141.681695
ACGATAT66850.0141.62335
Produced by FastQC (version 0.11.5)