FastQCFastQC Report
Sat 18 Mar 2023
SRR4895419_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895419_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1700969
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA82070.4824896867608992No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG36320.21352534937438603No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC31030.18242542927002198No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT28970.17031468533524127No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA27140.15955611183978072No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC23020.13533462397021934No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA18440.10840879522201756No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT18230.10717420482089914No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC17510.10294132344563597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT360800.0139.044461
GGTATCA361800.0138.441278
GTGGTAT362500.0138.034916
GTATCAA365650.0137.278959
TGGTATC366200.0136.758187
AGCAGTG368000.0135.952322
CAGTGGT370650.0135.096864
AGTGGTA370900.0134.947565
GCAGTGG375700.0133.070143
ATCAACG363050.028.16405310-14
CAACGCA364200.028.15024410-14
TCAACGC364600.028.09566510-14
AGAGTAC363750.028.05905315-19
CAGAGTA364800.027.95855515-19
GTACATG363750.027.85500520-24
TACATGG362800.027.82474120-24
AGTACAT366650.027.75251420-24
CGCAGAG368450.027.6737715-19
GCAGAGT370150.027.50387815-19
GAGTACA364250.027.39769720-24
Produced by FastQC (version 0.11.5)