FastQCFastQC Report
Sat 18 Mar 2023
SRR4895418_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895418_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1773741
Sequences flagged as poor quality0
Sequence length150
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA1774650.0143.919621
TAGCGAC1774800.0143.907462
AGCGACT657200.0143.799513
AGCGACG582100.0143.576163
GCGACTA200200.0143.529174
CGACTAG67550.0143.463765
GCGACAT198550.0143.452774
AGCGACA537450.0143.313493
GCGACTT250450.0143.076784
GCGACGT214400.0142.92214
GCGACTG208450.0142.78784
CGACGTA70550.0142.670015
CGACTAT74100.0142.636415
CGACTGA59450.0142.42235
GCGACGA168200.0142.41294
CGACGAT63000.0142.05395
CGACGTT77050.0142.034385
CGACATT76200.0142.012485
CGACTTA72950.0141.92415
CGACATG64500.0141.875815
Produced by FastQC (version 0.11.5)