FastQCFastQC Report
Sat 18 Mar 2023
SRR4895418_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895418_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1773741
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA94420.5323212351746957No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG46840.2640746309635962No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC31580.17804177723805223No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA20730.11687162894695449No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA18440.10396106308643707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT514950.0139.822371
GGTATCA518650.0138.247928
GTGGTAT518700.0138.23466
TGGTATC519950.0137.846887
GTATCAA523350.0137.44669
AGCAGTG522650.0137.294042
AGTGGTA523150.0137.072535
CAGTGGT524100.0136.824054
GCAGTGG531100.0135.028293
TAGCGAC1157.49221E-637.566022
ATCAACG520250.028.1928410-14
TCAACGC521750.028.16146710-14
AGAGTAC520250.028.11012515-19
CAGAGTA521250.028.05343415-19
TACATGG519950.027.85818120-24
GTACATG521550.027.8058520-24
CAACGCA530000.027.79917510-14
AGTACAT525700.027.7068720-24
GCAGAGT527400.027.65258615-19
CGCAGAG532800.027.49934815-19
Produced by FastQC (version 0.11.5)