FastQCFastQC Report
Sat 18 Mar 2023
SRR4895417_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895417_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences963252
Sequences flagged as poor quality0
Sequence length150
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCTAA963700.0143.925641
GGCTAAC964000.0143.880862
GCTAACG315900.0143.764983
GCTAACT365500.0143.67773
GCTAACA282550.0143.508763
CTAACTG122950.0143.407324
CTAACGT115050.0143.116794
CTAACGA93400.0142.913734
CTAACGG108350.0142.730384
TAACGTG38300.0142.11315
TAACGAG31150.0141.450495
CTAACTA109400.0141.360474
TAACTAG37600.0141.120685
TAACTGG43600.0141.020555
CTAACTT137300.0140.79424
TAACGGA30650.0140.70435
CTAACAT107400.0140.574074
TAACGGT43700.0140.533115
CTAACAG96400.0140.482684
TAACGAT34050.0139.764025
Produced by FastQC (version 0.11.5)