FastQCFastQC Report
Sat 18 Mar 2023
SRR4895417_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895417_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences963252
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52700.5471050150947No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT18690.1940302226208718No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG17260.1791846785680175No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT16970.17617404376009602No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC15690.16288572460789078No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA15460.1604979797602289No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT288900.0140.069931
GGTATCA291700.0138.464198
GTGGTAT292050.0138.298266
GTATCAA293800.0137.866589
TGGTATC293150.0137.73027
AGTGGTA293550.0137.591575
CAGTGGT294150.0137.286444
AGCAGTG294200.0137.179462
GCAGTGG295850.0136.438743
ATCAACG291800.028.2705710-14
AGAGTAC292250.028.1632815-19
TCAACGC293700.028.12690510-14
CAGAGTA293050.028.05691715-19
TACATGG290450.028.04268820-24
GTACATG292050.027.95808820-24
CAACGCA296550.027.92942410-14
GCAGAGT294550.027.82115215-19
AGTACAT296300.027.72231320-24
CGCAGAG297500.027.67112415-19
AACGCAG299650.027.60204310-14
Produced by FastQC (version 0.11.5)