FastQCFastQC Report
Sat 18 Mar 2023
SRR4895416_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895416_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1185976
Sequences flagged as poor quality0
Sequence length150
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCATG1187300.0143.830961
GCCATGC1187750.0143.776462
CCATGCG409100.0143.501923
CCATGCT424600.0143.40123
CATGCTA122000.0143.345524
CATGCGT144050.0143.344944
CCATGCA355500.0143.204833
CATGCTG138850.0143.165054
CATGCGA115700.0142.812364
CATGCAT131000.0142.73064
CATGCGG149950.0142.602284
CATGCAG121900.0142.44
ATGCGAT41450.0141.910325
CATGCTT165150.0141.596954
ATGCGAG38800.0141.582415
ATGCGGT60000.0141.234795
ATGCGAA36400.0140.829975
ATGCGTG46850.0140.767495
ATGCGTA45950.0140.704285
CATGCAA104900.0140.63164
Produced by FastQC (version 0.11.5)