FastQCFastQC Report
Sat 18 Mar 2023
SRR4895416_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895416_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1185976
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50350.4245448474505386No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG29980.2527875774889205No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA20850.17580456940106715No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC20550.17327500725141148No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT17380.14654596720338353No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA16290.13735522472630138No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT15540.13103131935216228No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT383100.0140.0321
GGTATCA386450.0138.27788
GTGGTAT386550.0138.260676
TGGTATC387900.0137.705257
GTATCAA389400.0137.655529
AGTGGTA389150.0137.336915
CAGTGGT390100.0137.07634
AGCAGTG390200.0137.041152
GCAGTGG394650.0135.495913
ATCAACG387300.028.20833610-14
TCAACGC389350.028.12268610-14
CAACGCA391400.028.05632810-14
AGAGTAC389850.028.00535815-19
CAGAGTA390450.027.95494515-19
TACATGG388300.027.93914420-24
GTACATG389600.027.8828820-24
GCAGAGT390650.027.87428315-19
CGCAGAG393100.027.75916715-19
AGTACAT393300.027.71942120-24
AACGCAG396100.027.70888110-14
Produced by FastQC (version 0.11.5)