FastQCFastQC Report
Sat 18 Mar 2023
SRR4895415_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895415_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences835279
Sequences flagged as poor quality0
Sequence length150
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACCT837200.0143.659941
GCACCTC838250.0143.482
CACCTCG281750.0142.69213
CACCTCA260600.0142.475813
ACCTCGG98300.0142.310754
ACCTCGA81850.0142.14814
ACCTCTA90250.0142.000934
ACCTCAG82750.0141.907184
CACCTCT301750.0141.752493
ACCTCGT102450.0141.74654
ACCTCAT99150.0141.308584
ACCTCTG99000.0140.795434
CCTCGAT30650.0140.471795
CCTCGTA34050.0140.189295
ACCTCAA80850.0139.631844
CCTCGAA27100.0138.681855
CCTCAGT31750.0138.326225
CCTCTGA29850.0138.206595
CCTCGGA28700.0137.974625
ACCTCTT115550.0137.826774
Produced by FastQC (version 0.11.5)