FastQCFastQC Report
Sat 18 Mar 2023
SRR4895415_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895415_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences835279
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36680.43913470828310064No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG29980.35892198894022237No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC23080.2763148600647209No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA22410.2682935881304331No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT20700.24782138662650444No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT11170.13372777239700748No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA8820.1055934603886845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA168100.0138.04488
GTGGTAT168350.0137.83986
AAGCAGT168950.0137.520751
GTATCAA170250.0136.682119
TGGTATC170700.0135.773477
AGCAGTG171200.0135.418982
CAGTGGT171650.0135.18984
AGTGGTA172000.0134.872855
GCAGTGG176150.0131.613573
ATCAACG168150.028.16595310-14
AGAGTAC168400.028.06428115-19
CAGAGTA168450.028.03030615-19
TACATGG168100.027.87451220-24
TCAACGC170300.027.82727610-14
GTACATG168650.027.8177620-24
CAACGCA171000.027.77230810-14
AGTACAT171250.027.53836620-24
GCAGAGT171400.027.43865215-19
GAGTACA168800.027.392120-24
ACATGGG171300.027.27814120-24
Produced by FastQC (version 0.11.5)