FastQCFastQC Report
Sat 18 Mar 2023
SRR4895414_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895414_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1645881
Sequences flagged as poor quality0
Sequence length150
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA75960.4615157474932878No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC18230.1107613490890289No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGTAC1647150.0143.880042
AGATGTA1649150.0143.705541
ATGTACG571700.0143.527713
ATGTACA451900.0142.910313
TGTACGA163200.0142.758444
GTACTAG62900.0142.505685
GTACGGT77450.0142.413385
TGTACTA176400.0142.279464
TGTACGG204100.0142.265184
GTACGAT63400.0142.176715
TGTACTG209950.0141.764684
TGTACGT208100.0141.675674
GTACTAA48100.0141.598795
GTACGTA60950.0141.158695
TGTACAG160650.0141.080644
TACGGTA23000.0140.863396
GTACGAA49600.0140.800285
GTACTTG88050.0140.722965
GTACGAG51700.0140.651475
GTACTAT65950.0140.609445
Produced by FastQC (version 0.11.5)