FastQCFastQC Report
Sat 18 Mar 2023
SRR4895414_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895414_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1645881
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG109920.6678490121703817No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA102020.6198504023073357No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG80410.4885529391250035No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC66900.4064692404857946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT760700.0135.500321
TGGTATC775700.0132.353477
GGTATCA776850.0132.222418
AGCAGTG776900.0132.202162
GTGGTAT777200.0132.172136
GTATCAA782350.0131.771419
CAGTGGT780800.0131.54434
AGTGGTA788000.0130.388055
GCAGTGG791950.0129.631333
GGTATAA7700.029.9205028
GCAGTTG13900.027.9722983
CAACGCA777050.027.21225510-14
ACATGGG760100.027.17342620-24
ATCAACG776300.027.10685210-14
TCAACGC779500.027.04914710-14
AGAGTAC777500.027.04464315-19
TACATGG773450.027.00786420-24
CGCAGAG779500.026.93461615-19
GTACATG776700.026.9226620-24
CAGAGTA781450.026.90241415-19
Produced by FastQC (version 0.11.5)