FastQCFastQC Report
Sat 18 Mar 2023
SRR4895413_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895413_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2833772
Sequences flagged as poor quality0
Sequence length150
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATTCG950350.0143.827843
CAGATTC2837150.0143.821932
ACAGATT2841150.0143.619461
GATTCGA268400.0143.484834
AGATTCA853200.0143.167533
GATTCGG302050.0143.041054
AGATTCT1038950.0142.989673
GATTCGT383200.0142.735674
GATTCTA309650.0142.645924
GATTCAG286350.0142.611624
GATTCAT317100.0142.405154
GATTCTG308150.0141.915074
ATTCGAG89000.0141.81035
ATTCGAT103850.0141.706675
ATTCGAA78950.0141.16755
GATTCAA255250.0140.835344
ATTCGTA113300.0140.563035
ATTCGGA85250.0140.362965
GATTCTT432300.0139.780914
ATTCGGT119250.0139.345615
Produced by FastQC (version 0.11.5)