FastQCFastQC Report
Sat 18 Mar 2023
SRR4895413_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895413_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2833772
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA216920.7654814854547226No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG200730.7083491544132696No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC111900.39488003974914No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT39620.13981364767525403No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTTTCAAAGTGGAGAACC38090.13441448359289315No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA35030.12361615542817135No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT33390.1178288161503466No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA30750.10851261145921408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT919750.0139.708571
TGGTATC930100.0137.601887
GGTATCA932500.0137.317238
GTGGTAT932850.0137.211696
GTATCAA940950.0136.535529
AGCAGTG939550.0136.304582
CAGTGGT940400.0136.133044
AGTGGTA946050.0135.35815
GCAGTGG954250.0134.136953
CAACGCA932700.028.24029410-14
ATCAACG930450.028.1956110-14
TCAACGC933200.028.15109610-14
CGCAGAG936950.027.95111815-19
AGAGTAC941950.027.79969615-19
TACATGG935050.027.73978820-24
CAGAGTA944850.027.70522715-19
ACATGGG928250.027.68702720-24
AACGCAG951300.027.68662310-14
GTACATG940200.027.65370220-24
GCAGAGT944250.027.6465815-19
Produced by FastQC (version 0.11.5)