FastQCFastQC Report
Sat 18 Mar 2023
SRR4895412_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895412_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1838500
Sequences flagged as poor quality0
Sequence length150
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAC1842550.0143.678791
ACGACCT654050.0143.621023
CACGACC1843450.0143.608642
ACGACCG627850.0143.53663
CGACCTA196200.0143.481554
ACGACCA558500.0143.363633
CGACCAT191900.0143.319964
CGACCGT228850.0143.177324
CGACCGA177900.0142.943054
GACCTAT69450.0142.543935
CGACCAA169400.0142.422734
GACCGTA67150.0142.387015
GACCTAG71100.0142.37515
CGACCTG226000.0142.243134
CGACCAG198900.0142.18544
GACCGAT66500.0142.154755
CGACCTT235700.0142.101414
GACCATG68600.0141.896225
CGACCGG224350.0141.65264
GACCAGT75450.0141.418845
Produced by FastQC (version 0.11.5)