FastQCFastQC Report
Sat 18 Mar 2023
SRR4895411_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895411_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2024843
Sequences flagged as poor quality0
Sequence length150
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTTGC2028900.0143.704562
AATGTTG2035650.0143.228061
TGTTGCA571300.0142.621893
GTTGCGA184000.0142.15654
TGTTGCG724700.0141.919163
GTTGCAG195050.0141.817724
GTTGCAT211650.0141.716384
GTTGCTA213900.0141.605734
GTTGCAA168350.0140.830834
TGTTGCT760550.0140.379333
TTGCGAT69200.0139.625755
GTTGCGG289650.0139.42194
GTTGCGT259250.0139.413244
TTGCGGA67750.0138.682075
TTGCTAG75650.0138.570745
TTGCGTA76500.0138.53695
GTTGCTG268600.0138.420144
TTGCGAA55900.0138.199665
TGCGGTA38950.0138.080466
TTGCAGT78450.0136.928855
Produced by FastQC (version 0.11.5)