FastQCFastQC Report
Sat 18 Mar 2023
SRR4895411_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895411_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2024843
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA114160.5637967980727395No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTCCCGACCCGGGGAGGTA54510.26920605696342875No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTGGAGGGCAAGTCTGGTG51210.2529084971032322No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGACTCTTTCGAGGCCCTG47300.23359835799615083No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCCGCGGTAATTCCAGCTC43880.21670815959558345No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGTCTGGTGCCAGCAGCCG35660.17611241958018473No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCGCGCAAATTACCCACT34720.17147008434728025No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCTCCAATAGCGTATATTA34650.17112437853206397No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA33910.16746977419977746No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGAAAAAAAAAAAAAAAA28600.14124551878837024No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACGAGGATCCATTGGAGGG27990.13823293954148544No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTTTAAATCCTTTAACGAG26240.12959029416107815No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGAGGTAGTGACGAAAAA26020.1285037901703984No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG24580.121392127685949No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA24420.12060194296545461No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGCTGCCCTCTCCTGGGC23860.11783629644372429No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGTGACCACGGGTGACGG22190.1095887434235642No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT21710.10721818926208106No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCATGATCTTGTTACTGT21200.10469947546550523No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGGTTCGATTCCGGAGAGG21110.10425499656022713No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGCCGCGGTAATTCCAG20650.10198321548880579No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT906500.0140.617951
AGTGGTA922400.0137.827165
AGCAGTG922800.0137.728422
TGGTATC923600.0137.64037
GTGGTAT923750.0137.633536
GGTATCA925000.0137.502038
CAGTGGT925300.0137.387424
GTATCAA929900.0137.180089
GCAGTGG928750.0136.79183
TGTATCA12500.032.2545248
GTTGTAT13300.030.855736
ATCAACG925350.028.12177810-14
TCAACGC927500.028.10627210-14
CAACGCA932600.028.0220510-14
AGAGTAC929100.027.95558215-19
CAGAGTA929250.027.95106915-19
AACGCAG936050.027.9095410-14
TACATGG925200.027.823420-24
CGCAGAG935200.027.79633115-19
GCAGAGT932850.027.75984815-19
Produced by FastQC (version 0.11.5)