FastQCFastQC Report
Sat 18 Mar 2023
SRR4895410_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895410_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1022883
Sequences flagged as poor quality0
Sequence length150
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCGTC1023450.0143.911132
AATCCGT1023900.0143.847891
TCCGTCG347350.0143.187183
TCCGTCA309300.0143.157563
CCGTCGA103600.0142.814124
CGTCGTA42550.0142.472695
CCGTCAG101450.0142.434254
TCCGTCT373050.0142.335783
CCGTCTA115700.0142.31544
CCGTCAA92950.0142.136554
CCGTCAT116500.0141.956134
CGTCGAA33200.0141.826935
CGTCGAT38900.0141.589465
CCGTCGT132900.0141.395174
CGTCTAG40350.0141.319065
CGTCAAG31250.0141.230855
CGTCAGT40300.0140.779775
CGTCTTA38950.0140.66835
CGTCTAA33500.0140.556855
CGTCAAT35150.0140.513445
Produced by FastQC (version 0.11.5)