FastQCFastQC Report
Sat 18 Mar 2023
SRR4895410_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895410_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1022883
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49630.4851972317459573No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG29450.28791171619823575No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT20780.20315128905260912No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC19020.18594502010493869No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA18940.18516291697095366No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT18360.17949266924956228No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA12370.12093269709243383No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC11510.11252508840209487No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA11370.11115640791762108No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGAAAAAAAAAAAAAAAA10350.10118459295931208No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT253950.0138.849171
GGTATCA255300.0137.734828
GTGGTAT255350.0137.51056
GTATCAA257150.0137.247919
CAGTGGT257250.0136.578834
AGCAGTG257250.0136.536212
TGGTATC257400.0136.52727
AGTGGTA257950.0136.180285
GCAGTGG260950.0134.600253
ATCAACG255600.028.17940110-14
TCAACGC257000.028.0763210-14
AGAGTAC255250.028.06008315-19
CAACGCA258850.027.9702310-14
CAGAGTA256450.027.95685815-19
TACATGG254900.027.92349220-24
GTACATG256150.027.79284720-24
GCAGAGT258300.027.6785815-19
CGCAGAG260250.027.61504415-19
AGTACAT258800.027.60284820-24
AACGCAG263250.027.4917910-14
Produced by FastQC (version 0.11.5)