FastQCFastQC Report
Sat 18 Mar 2023
SRR4895409_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895409_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences522845
Sequences flagged as poor quality0
Sequence length150
%GC29

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGACA524400.0143.552441
AGGACAC524850.0143.429372
GGACACG164800.0143.118043
GACACTG63850.0142.751454
GGACACT202250.0142.603473
GACACGT60550.0142.446034
GGACACA159650.0142.36833
GACACTT77700.0141.953284
GACACGG55250.0141.646224
GACACGA49950.0141.541464
GACACAT59300.0141.199364
GACACAG51050.0141.030154
GACACTA61500.0140.596854
ACACTGG22750.0139.561255
ACACTAG21200.0139.237325
ACACGGA17450.0139.040775
ACACTTG26000.0138.730535
ACACAGT21600.0138.658745
ACACGTA20100.0138.618945
ACACGGT20850.0138.466925
Produced by FastQC (version 0.11.5)