FastQCFastQC Report
Sat 18 Mar 2023
SRR4895409_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895409_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences522845
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22410.4286165115856516No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA21230.40604768143522457No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT20680.395528311449856No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG9390.1795943348411097No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTCTTGCTTCAAC7330.14019451271409308No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC7320.14000325144163184No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT6610.1264237010968834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT119750.0139.906561
GGTATCA121650.0137.603048
GTGGTAT121800.0137.197136
AGCAGTG122600.0136.243162
GTATCAA123150.0136.219319
TGGTATC123150.0135.810077
CAGTGGT123500.0135.30864
GCAGTGG123650.0135.144453
AGTGGTA124550.0134.167915
ATCAACG122050.028.07930810-14
AGAGTAC122500.027.94142715-19
CAGAGTA122900.027.85048915-19
TACATGG121700.027.8432620-24
CAACGCA124600.027.71266610-14
TCAACGC124400.027.66462110-14
GTACATG122900.027.64170320-24
GCAGAGT123700.027.57724415-19
AGTACAT124100.027.46725320-24
TGCGACT1050.00797113827.4276055
CGCAGAG125350.027.35209315-19
Produced by FastQC (version 0.11.5)