FastQCFastQC Report
Sat 18 Mar 2023
SRR4895408_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895408_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2443733
Sequences flagged as poor quality0
Sequence length150
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAA2444750.0143.928052
GCGAGTA2445250.0143.898621
GAGTAAG859300.0143.361533
GAGTAAT903150.0142.977523
GAGTAAA695800.0142.499913
AGTAAGG315350.0140.085944
AGTAATG328900.0139.918434
AGTAAGA240750.0139.564034
AGTAAGT324000.0139.412434
AGTAATA264850.0139.069724
GTAATGA88650.0137.168035
GTAATAG98350.0137.035615
GTAAGTA95150.0136.953085
AGTAAAT267550.0136.616844
GTAATTG127200.0135.896135
AGTAAAG266150.0135.739494
GTAAGGA90200.0135.688925
AGTAATT339450.0135.527394
GTAAGTG115250.0135.306785
GTAATTA95700.0134.059545
Produced by FastQC (version 0.11.5)