FastQCFastQC Report
Sat 18 Mar 2023
SRR4895408_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895408_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2443733
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA108230.442887991445874No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG65790.26921926413401137No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC51480.21066131201731123No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT793100.0139.661831
GTGGTAT799900.0138.00376
GGTATCA800750.0137.938138
TGGTATC800750.0137.920157
GTATCAA806950.0137.270879
CAGTGGT809450.0136.38444
AGCAGTG810000.0136.321262
AGTGGTA812250.0135.931995
GCAGTGG818200.0134.893453
ATCAACG801350.028.1904710-14
TCAACGC802700.028.189710-14
CAACGCA806100.028.14403310-14
AGAGTAC804400.028.04679515-19
CAGAGTA807600.027.93209615-19
TACATGG804400.027.83281720-24
GTACATG806900.027.80012120-24
CGCAGAG811550.027.76243415-19
AGTACAT812000.027.71064220-24
GCAGAGT811350.027.7000615-19
ACATGGG808500.027.52958520-24
Produced by FastQC (version 0.11.5)