FastQCFastQC Report
Sat 18 Mar 2023
SRR4895407_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895407_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1290969
Sequences flagged as poor quality0
Sequence length150
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGATC1292250.0143.847522
GAGATCG421550.0143.633713
GAGATCA378050.0143.554343
GAGATCT493600.0143.525623
AAGAGAT1295500.0143.486651
AGATCGT165450.0143.165574
AGATCGA126600.0143.082474
AGATCTA146400.0142.910464
AGATCAT142500.0142.77984
AGATCTG155300.0142.69434
GATCGAT48900.0142.520055
AGATCTT194200.0142.435294
AGATCAG119100.0142.420674
GATCGTA49550.0142.248785
AGATCAA118550.0142.170464
GATCTAG47100.0142.005225
GATCAAG38350.0141.739565
GATCTAT56250.0141.688495
GATCATA40450.0141.678535
GATCGAA37950.0141.526115
Produced by FastQC (version 0.11.5)